A toothgel containing dichloromethane extract of mangosteen pericarp (Garcinia mangostana L.) had been formulated as an antiplaque. The aims of this research was to determine the effectiveness of the dichloromethane extract of mangosteen pericarp as an active substance in toothgel againts bacteria of plaque formation had been done. Toothgel physical stability test carried out for 4 weeks include organoleptic, homogeneity, foam ability, spread ability, viscosity, and pH during storage in low temperature (40 ± 20C), room temperature (290 ± 20C) and high temperature (400 ± 20C). The results of the stability test showed that all formulas no change occurred, but viscosity, pH, and mangosteen derivate concentration, change occurred. Agar diffusion method was used to determine the antibacterial effectivity againts bacteria isolated from plaque. There were four of Bacterial isolates include Streptococcus, Streptobacillus, Monococcus, and Staphylococcus. Toothgel effectivity test group being used are negative control (F0), positive control (X ® herbal toothgel), and F1, F2, and F3 concentrations of dichloromethane extract of mangosteen pericarp is 0.1; 0.5 and 1.0% by using carbopol 940P as a gelling agent. The result of the effectivity test were analyzed statistically by SPSS 17.0 using One-Way ANOVA and continued to Duncan Multiple Range T-Test showed a significantly different at probability <0.05; it is suggested that the increased concentrations of dichloromethane extract of mangosteen pericarp affect toothgel antibacterial activity significantly.
