Der Pharmacia Lettre
Abstract
Author(s): Gajanan Shinde
Methoxsalen is powerful Anti-psoriatic agent , widely used in the treatment of Psoriasis and also used in vitiligo.
The pharmacokinetic parameters of Methoxsalen make it a suitable candidate for development of Nanostructured
Lipid Carrier (NLC). The purpose of the present investigation was to prepare and evaluate Methoxsalen loaded
nanostructured lipid carriers based gel. Methoxalen loaded NLC were prepared by hot homogenization followed by
High speed homogenization technique, using Glyceryl distearate, Compritol ATO 888 and Stearic acid as solid
lipid, Ethyl oleate as liquid lipid and Pluronic F-68 as surfactant. NLC were characterized for particle size,
polydispersity index (PDI), zeta potential, entrapment efficiency and In vitro release. The effect of composition of
lipid materials and surfactant on the particle size, polydispersity index (PDI), zeta potential, entrapment efficiency
and In vitro release were investigated. DSC analysis was performed to characterize the state of drug and lipid
modification. Shape and surface morphology was determined by Scanning electron Microscopy (SEM). NLC based
gel was characterized for pH, Viscosity, Spredability, Refractive Index, Drug content, Skin permeation, Drug
deposition and Skin irritation study. NLC based gel Formulations were subjected to stability study over a period of
30 days. The mean particle size, polydispersity index (PDI), zeta potential and entrapment efficiency and In vitro
release were found to be 258.2 nm, 0.183, -20.9 mV, 97.45 % and 53.75 % respectively. DSC studies revealed that
Methoxsalen was compatible with other ingredients. Shape and surface morphology was determined by SEM
revealed fairly spherical shape of nanoparticle. In vitro release studies demonstrated that NLC formulations
possessed a controlled release over a period of 24 hrs. The pH, Viscosity, Spredability, Refractive Index, Drug
content, Skin permeation, Drug deposition were found to be 6.8, 2499.49 cps, 5 cm, 1.3671, 98.05 %, 50.32 % and
29.64 %. NLC based gel was not shown skin irritation and shown prolong release up to 24 hr. There was
insignificant change in pH, Drug content and In vitro release indicating the developed NLC were fairly stable.
Fairly spherical shaped, stable and controlled release Methoxsalen-NLC based gel could be prepared by hot
homogenization followed by High speed homogenization technique.
