The present study was undertaken within a year from January 2012 to December 2012 in different localities of coniferous forests of Rajouri Dist. of J&K, India to collect the samples of macro fungi. The vegetation and climatic conditions of state Jammu and Kashmir in India possessed a prime location in the world for the hot spot and biodiversity of macro-fungi. Although this region is still unexplored due to unawareness and less attention towards this subject. The study was therefore done to explore the variable and diverse species of macrofungi which have socio-economic and medicinal importance. The present study is describing the systemic position and taxonomic identification of five new genera of macrofungi which were not explored previously. The five new species of macrofungi that were explored and taxonomically identified are Scleroderma citrinum, Psilocybe subtropicalis, Ganoderma applanatum, Cyptotrama asprata and Entoloma serrulatum. The voucher specimens after taxonomic identification were deposited in NCFT, New Delhi, India and were assigned axccession numbers.