Nitraria schoberi (Zygophyllaceae) is native to the desert regions of Mighan in Arak of Iran. Also because many alkaloids have many biological properties. Among the properties that can contract effect on blood vessels called action and relief providers, and resistance to drought, salinity and contamination of the plant, the results indicate that is resistant to drought and salinity tolerance to pollution CuSo4 at a concentration of 600 mg/m3. In study Nitraria schoberi of family Nitrariaceae and Spindale's order, the total amount of alkaloids in different organs during different phonological were studied. The aim of different organs (leaf, root and branch) and (leaf, roots, stems, flowers) during the flowering stage and (leaf, roots, stems, crown of branch, fruits) during the fruiting stage, at various times, were collected from the desert region of Mighan Arak of Iran. After extraction, using three methods: Soxhlet, ultrasonic and reflux, the total amount of alkaloids in different organs using spectroscopic and gravimetric methods were extracted. The results showed that the different of mean total alkaloids in organs of the plant (leaf, stems, roots, flowers, crown branch, fruits) in all courses is significant (p<0.01) and leaf of plant, are more alkaloids than other organs. Among the different phonological period fruiting period, much more alkaloids in all organs, other than the period indicated. The alkaloid production in the fruiting period to reach its highest. The alkaloid obtained from the gravimetric method is higher than the spectroscopic method, because, some alkaloids no complex formation with BCG, therefore, spectroscopic methods showed lower levels of alkaloids.