The present investigation was carried out to revive the old art of dyeing with natural dye from barks of Odina wodier.L. Odina wodier.L belongs to family Anacardiaceae, commonly known as votiyar tree. The dye has good scope in the commercial dyeing of cotton in garments industry. In the present study, bleached cotton fabrics were dyed with chemical and natural mordants. Dyeing was carried out by pre-mordanting, post mordanting and simultaneous mordatning. The dyed samples have shown good washing, light, rubbing fastness and perspiration fastness properties properties. The various colour changes were measured by computer colour matching software. ICPMS studies have proved that, heavy metals such as antimony, arsenic, cadmium and lead were not present in the dye extract. Anti-bacterial and anti-fungal activities of the dye were also studied.