Annals of Biological Research
Abstract
Author(s): Nguyen Hoang Loc, Nguyen Thi Quy Hoa, Pham Thi Kim Cuc, Hoang Tan Quang
We cloned a gene coding chitinase (chi42) from strains Trichoderma asperellum SH 16 isolated from Thua Thien
Hue province, Vietnam. Expression of the chitinase in Saccharomyces cerevisiae INVSc1 produced a fusion protein
of approximately 50 kDa (42 kDa of mature chitinase, 4 kDa of signal peptide and 3.4 kDa of fusion tag of
pYES2/NT(C)/chi42 vector). The effect of induction time on the chitinase production was investigated. The results
showed that the highest total activity (0.012 unit/mL) of chitinase occurred after 12 h of induction with 2%
galactose.
