Prostaglandin (PG) secreted is characteristic for most inflammatory diseases. The committed step in the form of free arachidonic acid into PG products is supported by cyclooxygenase (COX) which exists as two genetically distinct iso forms, COX-1 and COX-2. This study was conducted using expolysacchaide produced by isolated strain paenibacillus, named PLEPS. Strain NRC1 was isolated from mangroves and identified as belonging to paenibacillus lactes NRC1 species by 16SrRNA and it produced 9.5 g of PLEPS per liter of broth medium. The PLEPS was a heteropolysaccharide composed of arabenose, fructose, glucose and galactouronic acid at molar ration 3.0: 1.0: 0.25: 3.0, respectively with molecular mass of 2.55´104 g/mol. To verify the claims of PLEPS on the anti-inflammatory activity and possibly deduce its activities, through testing its effect as cycoloxygenases 1 and 2 inhibitor at five concentrations was tested. It inhibited each of them concentration dependently to reach maximum activity, 98.82 %, for COX-2 and 55.18% for COX-1 at 400 μg/mL. However, celecoxibe inhibited them by 100% and 69.33%, respectively at the same concentration. PLEPS scavenged 50% of nitric oxide radical at 50.03 μg/mL, DPPH radical at 25 μg/ ml and superoxide radicals at 17.73 μg/mL. In in-vivo study, PLEPS inhibited production of interleukin-1 (IL-1), TNF-α and nitric oxide in sera of animals pretreated with PLEPS at 200 mg/ kg body weight before carrageenan injection in acute inflammation model. It is evident from recorded activities that it can be used as a potential antioxidant and Non-steroidal anti-inflammatory material through enhances adaptive immune responses.
