Polianthes tuberose L. is one of important cut shoot flowers in Iran and whole of world. Notwithstanding this flower has high potential after harvesting but, it is wilting very soon in houses. One of important new methods to improve quality and quantity of cut shoot flowers is use the preservative solution of flower. For this purpose, a randomized block experiment design with three treatments, four concentration and three replicates was established for increasing lifetime and quality of Polianthes tuberose L. in the greenhouse under uncontrolled environmental conditions. Three preservative solutions of Cobalt chloride, 8-hydroxyqunoline sulphate and ammonium sulphate with saccharose 5% were used. In this study, most amount of dry matter was in treatment of interaction effect of Cobalt chloride (800 ppm) with 8- hydroxyqunoline sulphate (0 ppm) and ammonium sulphate (0.2%). Maximum electrical conductivity of preservative solution was in treatment of interaction effect of Cobalt chloride (600 ppm) with 8-hydroxyqunoline (200 ppm) and ammonium sulphate (0%). Also, most amount of measured brix was in treatment of interaction effect of Cobalt chloride (600 ppm) with 8- hydroxyqunoline (400 ppm) and ammonium sulphate (0%). Maximum lifetime of flowers was in treatment of interaction effect of Cobalt chloride (0 ppm) with 8-hydroxyqunoline (600 ppm) and ammonium sulphate (0.4%).