In this study, have been evaluated the effect of four citrus rootstocks [(Sour orange (Citrus aurantium), Bakraei (C. reticulate × C. limmetta), Mexican lime (C. aurantifolia) and Volkamer lemon (C. volkameriana)] Valencia orange leaf chlorophyll content and mineral elements concentration (N, P, K, Ca, Na, Mg, Fe, Zn, Mn, Cl, Cu, and B) in completely randomized design with four replications. Budded Valencia orange on rootstocks were grown in pots containing calcareous soil (pH=8.2) in greenhouse. Results showed that rootstocks had significant effect on leaf chlorophyll content. Highest chlorophyll content was on Sour orange rootstock. Rootstock types had significant effect on leaf mineral concentrations except of Cl and Na. Leaf N concentration on Mexican lime and Sour orange rootstocks was lower than optimum range. Leaf Cu concentration on all rootstocks except of Sour orange and Cl concentration on all rootstocks were higher than optimum range. Scions on Volkamer lemon rootstock had highest Cl and lowest Na concentrations.