The wound healing activity of a popular Nigeria traditional Polyherbal ( A. conyzoids, C. scandens and M. villosus) remedy was investigated. The ethanol leaves extracts were formulated in form of an ointment using palm kernel oil as base. Two, 4 and 6 g/ml of the extracts and their combination were prepared and applied topically on the wounds daily for 20 days. Cicatrine powder (neomycin-bacitracin) served as the standard while the control group received only palm kernel oil. Daily wound contraction and epithelization time was recorded for each group. Agar well diffusion method was used for the determination of antimicrobial activity of the extracts. Phytochemical analysis of the plant extracts revealed the presence of alkaloids, saponins, flavonoids and tannins. Ageratum conyzoids and C. scandens showed activity against all the tested microorganisms while M. villosus inhibited S. aureus, B. subtilis and P. aeruginosa. All the extract and their combination showed significant (p < 0.05) wound contraction activity compared with the control. No significant (p > 0.05) different was observed in the wound contraction activity of A. conyzoids and C. scandens compared with the standard from the 12th to 20th day. Also no significant (p > 0.05) difference was observed between A. conyzoids, C. scandens and extracts combination at 2 and 4 g/ml. Significant epithelization time was also observed for all the extracts compared with control. Based on our findings there is no justification for the traditional combination of the 3 plant extracts as equal effect as combination was observed especially in C. scandens and A. conyzoids.
