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Evaluation of the relation of antioxidant enzymes activity with drought tolerance in vegetative and reproductive growth stages | Abstract
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Abstract

Evaluation of the relation of antioxidant enzymes activity with drought tolerance in vegetative and reproductive growth stages

Author(s): Solmaz Shahandeh*, Ali Akbar Imani and Hossein Shahbazi

In order to evaluation of high yielding and high tolerance genotypes of wheat and activity relation of some antioxidant enzymes with drought tolerance in wheat genotypes, 10 wheat genotypes were cultured in a randomized complete block design with 3 replications under two condition of complete irrigation and drought tolerance in Agriculture and Natural Resources Research Station of Ardabil. The results of morphological study in this research indicate a high diversity of studied genotypes which high genetic diversity in different corrective actions (classic and traditional) can be useful. Drought stress decreased all traits, especially seed yield and number of fertile tillers. Based on the results of mean comparison and sensibility and drought tolerance indexes of genotypes 2 (C-80-4), 3 (Bloudan/3/Bb/7C*2//Y50E/3*Kal/4/MV17) and 4 (Yan7578 128/4 /Chil/24*Star) chosen as high yielding and high tolerance genotypes. In drought stress conditions, grain yield indicated significant correlation with grain weight per spike, days to flowering, days to maturity, seed weight, harvest index and STI, GMP and MP indexes and also showed negative and significant correlation with non-fertile tiller number, biological yield and sensibility indexes of TOL and SSL. In normal conditions, yield showed positive and significant correlation with its components and indexes STI and GMP, but did not show significant correlation with index trait. The interaction of stages (vegetative and reproductive) was significant at the level of 1% for the enzyme activity of Catalase, Ascorbate peroxidase and Superoxide Dismutase. There was also a significant difference at level of 1% between genotypes in terms of three enzymes activity. There was significant difference at the level of 1% between the interaction of stages in the genotype in terms of enzymes activity of ascorbate peroxides and superoxide dismutase. The enzyme activity of Catalase and superoxide dismutase increased in the reproductive stage than vegetative stage. According to results of principal component analysis and cluster analysis, second group include genotypes 2 (C-80-4), 3 (Bloudan/3/Bb/7C*2//Y50E/3*Kal/4/MV17) and 4 (Yan7578 128/4 /Chil/24*Star) which was more valuable in terms of all traits that evaluated except enzyme activity of catalase and ascorbate and were in the first rank in terms of yield in stress and normal conditions and stress tolerance and were recognized as a superior group.