Andrographis echioides Nees is a herbaceous plant, belonging to the family acanthaceae has been used for the treatment of fever, asthma and liver diseases. The objective of the study was to evaluate the phytonutrients in stem, root and leaf of A.echioides and thinlayer chromatography (TLC) profiling of sequential extracts of A.echioides. Proximate analysis includes moisture, ash, carbohydrate, protein and lipid content were determined. Vitamins (thiamine, riboflavin ascorbic acid and alpha tocopherol) and minerals (iron, calcium and phosphorus) were also analyzed. The leaf extract of A.echioides were prepared using different solvents which includes hexane, chloroform, acetone, ethylacetate, methanol and water. TLC profiling of all the extracts was studied. Among the various parts of A.echioides, leaves possess maximum amount of proteins, lipids and carbohydrates. Significant amount of iron, calcium and phosphorus was found to be present in A.echioides.Calcium was found to be maximum in root, stem and leaf (31.2, 61.2 & 74.8 mg/g) of A.echioides. Stem and leaf part of the plant contains higher amount of non enzymic antioxidants which includes thiamine, riboflavin, tocopherol and ascorbic acid. Relative front values of bands observed for the sequential extracts lies between 0.12 to 0.75 confirms the presence of phytochemicals which includes flavonoids, saponins, terpenoids, phenols and aminoacids.TLC profiling of all the extracts of A.echioides confirmed the presence of number of phytochemicals and different Rf values of phytochemical compound reflects an idea about their polarity. The results obtained in the present study indicated the whole plant, Andrographis echioides Nees as a rich source of phytonutrients, phytochemicals and minerals that contribute to its effectiveness as a traditional medicine.
