A novel, precise, rapid, economical, and Accurate HPLC method for evaluation of Octopamine in bulk and formulations was developed and validated. The Chromatographic resolution of Octopamine was achieved by using Acetonitrile: 0.01M Potassium phosphate (Ortho phosphoric acid ) Buffer , (10: 90 V/V) as a mobile phase UV detection at 273 nm and Sunfire C-18 column .flow rate 1ml/min. the extraction Recovery of octopamine from its formulation dosage form (tablets) was >99.10% And the calibration curve was linear (r2 = 1) over octopamine concentration ranging from 5 to 30 μ g /ml. the method has an accuracy of > 99% and LOD and LOQ of 0.03423 μ g /ml and 0.10680 μ g /ml respectively. A result of the present method was validated statistically and by recovery studies were found to be satisfactory.
