European Journal of Zoological Research
Abstract
Author(s): Kamrouz Kaboli, Peyman Bijanzad, Amid Reza Jeyrani Moggadam, Morteza Shahbazi
and Hossein Hosseini3
Mycoplasma gallisepticum (MG) is one of the most important diseases in Iranian poultry industry and all over the
world. Mortality, poor weight gain and increasing of feed conversion ratio (FCR) were seen in MG infected flocks,
and causes economical losses particularly in broiler chickens that were used for meat production. In this study,
rural broiler breeder farms located around east azarbaijan of Iran were investigated during 2011-2012. In each
farm 18 to 31 chickens were sampled. The prevalence of Mycoplasma gallisepticum was studied by ELISA test, and
then all positive cases were further examined by 16s rRNA methods. Our results indicated that the from 73 samples
were tested, 23 (31.50%) of samples were positive in ELISA. All seropositive samples were positive in PCR. It can
be concluded that ELISA serological tests should be only used as screening in monitoring programs to detect MG in
poultry flocks and positive results should be confirmed by routine microbiological tests. The higher rate of MG in
rural broiler breeder flocks indicated that these farms do not consider biosecurity and hygienic conditions.
