The present experiment was conducted to assess the anti-inflammatory property of essential oil of the aerial parts of Helichrysum italicum (Roth) G. Don against the denaturation of protein in vitro. The test oil, at different concentrations, was incubated with egg albumin under controlled experimental conditions and subjected to determination of absorbance to evaluate the anti-inflammatory property. Diclofenac sodium was used as the reference drug. The present results exhibited a concentration dependent inhibition of protein (albumin) denaturation by the test oil. The outcome of diclofenac sodium was found to be less effective when compared with the test oil. From the present findings it can be concluded that the Helichrysum italicum (Roth) G. Don essential oil possessed marked anti-inflammatory effect against the denaturation of protein in vitro. This effect could be either due to Polyphenolic content or to the synergistic activity rather than a single compound.
