In this study we tried to investigate the effect of chicory(Cichoriumintybus L.)on performance, egg quality, blood biochemical and immunity parameters of laying hens. This study was carried out with 500of Hy- line (W36) hens in 5 treatment groups,5 repetitions and 20 hens for each group. The groups were T1) Control group, T2, T3, T4, and T5 0.75%, 1%, 1.5%, and 2%chicory(Cichoriumintybus L.)powder respectively. The results showed that using this medicinal plant with different level had significant effects on performance, blood parameters and egg quality characters also show significantly effects on the immune system of laying hens.The best result for food intake and the highest production percent and shell weightwere seen in the group5. Alsothe serum total cholesterol,Triglycerides and LDL concentration were significantly reduced in groups of 4 and 5 compared to the control group (P<0.05). But the concentration of serum HDL and glucose were not significantly reduced in groups compared to the control group.