The elements present in medicinal plants play an important role in the treatment of diseases as described in Ayurveda. XRF technique is one of the most powerful and quick multi elemental analysis with high sensitivity. This technique has been applied for quantitative analysis of multielements present in dried leaves of Madhuca longifolia (Koenig) linn. Fifteen elements were detected. It indicates the association of 5 macro elements, 6 micro elements and 3 heavy elements.