Annals of Biological Research
Abstract
Author(s): Prem Raj Singh, Veena Sharma, Narotam Sharma, Devender Singh,
Jagdish Kandpal
Tuberculosis caused by Mycobacterium tuberculosis complex (MTC) is the leading cause of death worldwide.
Detection of these bacteria is generally done by acid fast staining which also stains Non-tuberculous mycobacterium
(NTM). It is significant to that this identification and differentiation between MTC and NMT be made before time,
as the drug sensitivity profile of NTM is different from that of Mycobacterium tuberculosis complex and NTM will
not responds to standard TB chemotherapy. In this study, we have evaluated a duplex polymerase chain reaction (DPCR)
to simultaneously detection and differentiation of Mycobacterium tuberculosis complex (MTC) and Nontuberculous
mycobacterium (NTM). The D-PCR described here is a rapid, important, and cost-effective tool for
determining whether the causative organism is Mycobacterium tuberculosis complex or Non-tuberculous
mycobacterium, and will be helpful for disease surveillance
