The study is an attempt to investigate diuretic activity of ethanolic extract of a polyherbal formulation of three drugs Bryophyllum pinnatum, Syzigium aromaticum & Ocimum sanctum by in-vivo method described by Lipschitz et al. (1943). In the present study, the extract of polyherbal formulation (Bryophyllum pinnatum, Syzigium aromaticum & Ocimum sanctum) was found to possess significant activity. This activity of polyherbal formulation extract may be attributed to their ability to sparks the excretion of Na+, K+ and Cl- concentration in urine along with increase in urinary volume.
