In the present study, Anxiolytic activity of hydro-alcoholic extract of Madhuca longifolia leaves (HAEML) was evaluated. The closed field test showed that after administration of hydro- alcoholic extract of Madhuca longifolia leaves (100 mg/kg) or diazepam (1 mg/kg), there was significant decrease in the number of rearing, assisted rearing and number of squares traveled compared to the control group which might be attributed to the anxiolytic activity of the plant extract. In the elevated plus maze test, hydro - alcoholic extract of Madhuca longifolia leaves (100 mg/kg, p.o.) showed significant (pÃ?â?¹Ã?â??0.05) increase in the time spent in the open arm and diazepam treated group showed more significant groups compare to control.