The anti-nutrient contents of the pulp, seeds and rind of Citrulluslanatus were evaluated. The study was carried out on both fresh and dried samples. Results of the investigation revealed that the anti-nutritional components such as saponin, alkaloid, hydrocyanic acid, phenols, oxalate, tannins, phytates were detected in all the samples but at a varying tolerable concentration. However, other anti-nutrient constituents were in small insignificant amount in all the parts of the fruit investigated. Although there were significant difference (p< 0.05) in the anti-nutrient compounds in the different parts of the fruit, seeds and rind which are the parts always discarded on the basis of acclaim toxicity, these can contribute immensely to recommended daily allowance and maintenance of good nutritional status and hence good health for both man and animals.