Adonis aestivalis Linn is a plant traditionally used for the cure and treatment of many ailments. The present study was aimed to investigate the Antilithiatic activity of extracts of dried leaves and roots powder of Adonis aestivalis Linn. The present study was concentrated on estimation of calcium, oxalate, phosphate, magnesium excretion in the urine; this elevated urinary calcium with high urinary oxalate might lead to calcium oxalate stone formation, following administration of extracts significantly lower urinary calcium and oxalate were observed, this enabled us to conclude that the extract is anti lithiatic activity.