In this study, different organic soluble extracts i.e. methanol, n-hexane, carbon tetrachloride and chloroform along with aqueous soluble fraction of both roots & leaves of Heliotropium indicum were designed to investigate for cell membrane stabilizing activity. An in vitro study of different organic soluble extracts stabilized erythrocytes’ (RBC) membrane significantly. The chloroform soluble fraction of roots revealed 31.59 % and 64.7 % whereas n-hexane soluble fraction of leaves demonstrated 29.27 % and 56.9 % inhibition of hemolysis of RBC caused by heat and hypotonic solution respectively. The assay result was compared with standard acetyl salicylic acid (0.10 mg/ml) which inhibited 35.26 % and 73.56% hemolysis of RBC caused by heat and hypotonic solution respectively
