In an experimental study, anti-bacterial activities of more than eighty soil inhabitant Penicillium isolates were evaluated. Isolates were cultured, simultaneously with bacteria: Pseudomonas syringae, P. viridiflava, Xanthomonas translucens, Agrobacterium tumefascines, Rathayiibacter iranicus, and one isolate of E.coli in PDB and the results were evaluated after one week. All tested isolates, had anti-bacterial activity against one or more bacterial strains tested. It seems that, most of the examined Penicillia, produce one or more antibacterial substances and are able to prevent growth and proliferation of bacteria in their habitat.