Rhodanese from Mortieralla isabellina, isolatedfrom polluted area, was produced using natural media based on treated cane molasses. The optimum conditions for the most significant factors was measured using statistical optimizationstrategy. Screening using Plackett-Burman was performed to determine the most significant cultural factors. Thereafter aquadratic model was used to optimize the condition for these factors. The crude enzyme was partially purified through precipitation by ammonium sulfate at different saturation levels. The peak of the enzyme activities was at 60-75% of amm. Sulfate. The optimum temperature for the enzyme activities was 30 to 40ºC. pH near neutrality was more appropriate for the enzyme activity. Michaelis-Menten kinetic parameters were determined using Lineweaver Burk plot. Km value for the partially purified was 0.46 mol/l/sec. There are no reports for using Mortieralla isabellina as rudeness producer.
