In four (4) areas (Bobo-Dioulasso, Boni, Fada-N’Gourma and Pobé-Mengao) belonging to three (3) regions of Burkina Faso, a survey of medicinal plants used by traditional healers for treatment of gastrointestinal parasites in both human and animals has been carried out from 2005 to 2006. Interview approach was used to document indigenous knowledge and uses of plant species as remedies. A total of 58 traditional healers constituted of 79.3% by men and 20.7% by womenwere interviewed for their knowledge on the use of plants for the treatment of gastrointestinal (GI) parasites. 12, 16, 18 and 12 respondents were met respectively at Bobo-Dioulasso, Boni, FadaN’Gourma and Pobé-Mengao. Twenty five (25) plants used in both humans and animals have been reported. 36 % of these plants are commonly used in all the 4 study sites for the treatment of gastrointestinal parasites. Results obtained showed that Cassia sieberiana, Anogeissusleocarpus, Danielliaoliveri, Balanitesaegyptiaca, Sabasenegalensis,Securidacalongepedonculata, Senna alataand Accassianilotica var. adansoniiwere the most cited plants. Cassia sieberiana, Balanitesaegyptiaca, Danielliaoliveri, Zizyphusmucronataand Accassianilotica var. adansoniiwere the most used plants for animals. Leaves and root bark were the main plant parts usedwhen decoction was the main mode of preparation of the remedies.