A sensitive, accurate and rapid reverse phase Liquid chromatography coupled tandem mass spectrometry (LCMS/MS) method was described to estimate Telmisartan in human plasma. Detection was made at m/z 513.2/469.3 for Telmisartan and 344/193.8 for internal standard using ESI Negative ion spray ionization mode. Analyst 1.5.1 software was used for the quantification. The stationary phase was Hypurity Advance C18, 50 X 4.6 mm, 5μm. The separation method developed produce recovery of 84.67%. Acceptable intra-day and inter-day precision (<15%) and accuracy (<10% diff.) were observed over the linear range of 2.901 to 330.015 ng/mL. The absence of any matrix effects was displayed. The retention time of analyte and internal standard was 1.38 and 1.41 minutes. The developed and validated method was successfully applied for bioequivalence and pharmacokinetic studies.
