Two simple, accurate, precise and reproducible spectrophotometric methods for the simultaneous estimation of Pioglitazone (PIO) and Glimipride (GLIM) have been developed. First method used was simultaneous equation method, in this two wavelengths (216 and 225 nm) were selected for the measurement of absorbance. Second method, absorption ratio method in which measurements are made based on the absorptivity at the isobestic point (228 nm) and absorption maxima of PIO (216 nm). The absorption maximum wavelengths of PIO, GLIM were observed at 216 and 225 nm respectively and the isobestic point at 228 nm. Linearity ranges from 5-25 μg/ml for both drugs. The proposed methods are recommended for routine analysis of PIO and GLIM as it is rapid, precise, accurate and reproducible. These methods were validated according to the ICH guidelines.
