Der Pharmacia Lettre
Abstract
Author(s): Gadi Raju Ramalinga Raju*, Raju V.K Vegesna and G S Prasad
A simple, precise, rapid and accurate RP- HPLC method was developed for the estimation of Frovatriptan
Succinate (FTP) in tablet dosage forms. An XTerra RP-C18 Column, 250x4.6 mm, column with 5 �Ž�¼m particle sizes
and the Mobile Phase consisting of 0.02M Potassium Dihydrogen Phosphate pH: 3.2 adjusted with Methanol &
Acetonitrile in ratio of 70:30 v/v & water: Acetonitrile (50:50 v/v) was used as a diluent in the gradient mode. The
flow rate was 0.8 ml/min and the effluents were monitored at 242 nm. The retention time was 3.066 min and the
detector response was linear in the concentration range of 10-120 �Ž�¼g/mL for FTP successively. The respective
linear regression equation being Y= 65647.112x + 45974.1647 for FTP. The Limit of Detection (LOD) & The Limit
of Quantification (LOQ) was found to be 1.00 & 3.00 �Ž�¼g/mL respectively for FTP. The percentage assay of FTP was
98.17%. The method was validated by determining its accuracy, precision and system suitability. The results of the
study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the
routine determination of FTP in bulk drug and in its pharmaceutical dosage forms.
