Endophytic fungal isolation and enumeration was carried out from different aged leaf varieties viz., young, mature, yellow, dry and infected of Acalypha indica in the Microbiology Laboratory, Department of Biomedical Engineering, Sathyabama University, Chennai-600119. Two methods viz., moist chamber and agar plate were used to isolate the endophytic fungi. Altogether 20 fungal species were isolated under 15 genera from both agar plate and moist chamber method from the aged leaves of Acalypha indica. Of which 14 species of 11 genera and 13 species of 10 genera were recorded from Agar plate and moist chamber respectively. Endophytic fungi isolated from Acalypha indica both in Agar plate and Moist chamber method were Alternaria alternata, Alternaria geophila, Botrytis cinerea, Wallemia sebi and white sterile mycelia. In Agar plate method, infected and dry leaf samples harboured more number of endophytic fungi followed by yellow. The dry and infected leaf samples contained the maximum number of fungal species. The fungus Geotrichum sp. and Helminthosporium sp. were predominant in moist chamber method.
