In order to locate the QTLs involved in the inheritance of callus induction and in vitro indices of drought tolerance in barley an experiment was carried out using a wheat-barley disomic addition lines. The results of analysis of variance revealed highly significant differences for callus primary fresh weight (CPFW), callus growth rate (CGR), callus relative fresh weight growth (CRFWG), callus relative growth rate (CRGR) and callus induction percentage (CIP) in the callus induction stage and CGR, RFWG, RGR, relative water content (RWC) and in vitro tolerance (INTOL) in the drought stress experiment indicating genetic variability and possible chromosomal localization of the genes involved in the genetic of callus induction and in vitro predictors of drought tolerance in barley. Mean comparison exhibited that most of the genes controlling callus induction and drought tolerance criteria are located on chromosomes 2H and 7H. The efficiency of added chromosome (EAC) indicated that chromosome 2H and 7H had the highest efficiency with positive effect for improvment of callus induction traits CPFW, CRFWG, CRGR and RFWG, RGR and INTOL in the drought stress stage, respectively.
