In order to determine the effect of zeolite mineral, bio-fertilizers and different levels of chemical nitrogen fertilizer under limited irrigation water management on peanut yield and its water use efficiency , a field experiment was conducted at Astaneh Ashrafiyeh province located at North of Iran. In 2010 growing season, Factorial split-plot in randomized complete block design with three replications was used. main plot was consisted of water regimes treatments (I1=dry land and I2=12 day Interval Irrigation) and sub factor was consisted of combination treatments of zeolite mineral (Z1= 0 and Z2=7 ton ha-1), bio-fertilizers and different levels of chemical nitrogen fertilizer (N1=0, N2= Azospirillum + Azotobacter, N3= 30 kg N ha-1+ Aazospirillum + Azotobacter, N4= 60 kg N ha-1+ Aazospirillum + Azotobacter and N5= 90 kg N ha-1+ Aazospirillum + Azotobacter) was performed. The results of this investigation showed that all traits (seed yield, pod yield, biomass yield and water use efficiency based on seed, water use efficiency based on pod and water use efficiency based on biomass) were significantly affected by applying the suggested treatments. The highest seed yield was found to be on 12 day interval irrigation (2561 kg ha-1), application of 7 ton ha-1 zeolie (2726 kg ha-1) and application of 30 kg N ha-1+ Aazospirillum + Azotobacter (2345 kg ha-1). The highest water use efficiency based on seed was found to be on 12 day interval irrigation (1.88 kg/m3), application of 7 ton ha-1 zeolie mineral (1.73 kg/m3) and application 30 kg N ha-1+ Aazospirillum + Azotobacter (2.03 kg/m3).