The characteristics of seeds obtained from nine varieties of wheat (Triticum aestivum) from different position were evaluated. A field experiment was set up in a farmer`s field at Coonalpyn, in 2010. Nine varieties of Triticum aestivum L. (cv BT-Schomburg, Excalibur, Genaro. Halberd, Janz, Machete, Molineux, Oxley, and Trident) and one of Triticum durum (Yallaroi) were sown in a randomized block design with four blocks. Resute showed that seed weight varied tremendously and the interactions of tiller order × seed position and genotype×seed position on the head were highly significant. Most seed position on the head of main tillers had heavier seed than their counterparts on second tillers, except the basal spikelet (BSPK) which had similar seed weight on both tillers. among all genotypes, Machete had larger seeds (38 mg/seed) and Janz and Molineux the smallest seed (28 mg/seed). Machete had heavier seed in all positions of the head than Janzand Molineux, but BT-Schomburg had similar seed weight in BSPK compared with Machete. Accumulation of nutrients in seed in different tillers did not follow a similar trend for all nutrients. Mg, P, and S content in seed on main tillers was greater in MSPK-OF compared to the same position on secondary tillers. Other nutrients showed different patterns of accumulation in seed from different positions on the main tiller. The amount of Mg, P and S were similar in seed from BSPK and USPK in both tiller orders. Also, Mg and P had similar content in seed of MSPK-OF in both types of tillers.