Annals of Biological Research
Abstract
Author(s): Ahmad Majd1, Celine Singh1*, Sadigheh Arbabiyan1, Fahimeh Salimpour1 and Seyed Abdolhamid Angaji2
Electromagnetic fields are an important environmental factor that can influence the growth and development of
plants. Exposure to EMF was performed by a locally designed EMF generator. Different treatments for dry and wet
seeds (from 2 mT to 4 mT for 30 to 120 minutes exposure time) were done. All the treatments showed significant
difference in comparison with control plants. The highest fresh and dry weight in both root and shoot had occurred
in plants grown from wet and dry pretreated seeds with 2 mT for 30 min, 2 mT for 120 min and the least amount had
occurred in plants grown from wet and dry pretreated seeds with 4 mT for 120 min (Respectively). For genetic
diversity DNA extraction and PCR with 12 RAPD primers were done. A total of 404 bands had generated by the 12
primers and out of that 110 bands were polymorphic bands. The primer showing maximum number of polymorphic
bands was UBC100. The RAPD analysis indicated that plants grown from wet and dry pretreated seeds with 4 mT
for 120 min showed genetic variation compared to control plants.
