Coleus scutellariodes [L] Benth (Miana or Mayana) is commonly used in Indonesian folk medicine to treat vaginal discharge. This study was aimed to evaluating effect of ethanolic extract of Miana leaf on IgM profiles in Balb/C mice with systemic vulvovaginal candidiasis. In experiment, 25 mice were divided into five groups, five mice per each group, for IgM serum analysis. Animals received a dose of 500 and 750 mg/kg of Miana extracts with orally and then intravaginal inoculated 1 x 10-2 C. albicans. Control vulvovaginal candidiasis group and positive group received 0.2 mL saline and ketoconazole (200 mg/kg), respectively, and inoculation. Normal group received 500 mg/kg of Miana extract and no infection with C. albicans. IgM level was measured at 0, 2, 3, 5 and 14 days by ELISA method with using albicans antibody kit according to the manufacturer’s protocol. The results indicated that the Miana-treated mice (dose of 750 mg/kg) produced lower IgM level than vulvovaginal candidiasis control groups at 0-14 days (p < 0.001) with values of 0.22-0.80 pg/mL and 0.25-1.74 pg/mL, respectively. This infected group showed similarly potency for IgM level in mice of ketoconazole positive control group (values, 0.22 to 0.78 pg/mL) at p < 0.001. Our results provide important information about the potential application of Miana ethanolic extract in the treatment of Balb/C mice with systemic vulvovaginal candidiasis
