In the recent decades development in studies in the field of immunology and hormonal by sport science researchers has increased. And also by the significant interaction between the immune and hormonal systems and physical activities, person will be affected by hormonal and safety changes so the interaction between systems and their changes after physical activities is important for the researchers and it can be useful for athletes health improvement and health of general population. As long as the subjects ofthe different groups of this research were rats, which were in a controlled environment and at a pre and post planned test, under the effects of independent variable [8-week exercise program], so there search method is experimental. In the present research, after the initial agreement, fourteen 3-month-old male Wistar rats were obtained from the Pasteur Institute Centre ofAmol. Variables measured in the exercise and control groups were compared and then the descriptive and inferential statistics were used to analyse the hypothesis test. Natural distribution of data measured by the Kolmogorov Simonov test, and the statistical analysis of the data performed using the software SPSS version 16 by the ANOVA test through repeated measurements based on the normal distribution. To compare variables between the two groups of the t test was used. The significant level for all calculations was considered as p <0.05. The results show that an eight-week endurance training can be considered as an effective factor for the immune and humoral system. Endurance exercise seems to be effective on plasma levels of IL-6 in male rats. The results of this study can be considered as a potential candidate to change the size of IL-6 in plasma of rats, and despite minor differences in the effects of endurance exercises on insulin of male Wistarrats, these results should be carefully considered and analyzed. ________
