An experiment was conducted to study the effect of different level of canola oil on the abdominal fat omega 3 deposition fatty acid profiles in Iranian native turkeys. A total of 90 turkey chicks were randomly divided into 3 experimental treatments with 3 replicates were arranged in a completely randomized design. The experimental period lasted 20 weeks. Experimental diets consisted of: Basal diet with 0% canola oil; basal diet with 2.5% canola oil and basal diet with 5% canola oil. Results show that canola oil could change n-3 fatty acids composition and significantly increased compared with control group, this status have beneficial effects on the increase omega 3 fatty acids of native turkeys and this status could help to enrichment of meat products.