Thyroid hormones have significant effect on the metabolism of the body, so that the complete lack of thyroid secretion may cause decrease inthe basic metabolic rate and, on the other hand, the excessive secretion of it can increase the basic metabolism. Results of previous studies have shown that regular aerobic exercise is an important factor in the regulation of thyroid hormones; therefore, in this study, the effects of 12 weeks aerobic exercise on plasma level of TSH and thyroid hormones, have been investigated in sedentary women. Thirty inactive obese women were randomly selected though purposive sampling and divided into two groups: aerobic exercise and control. The training program consisted of 12 weeks moderate-intensity aerobic exercises with music. These exercises were performed three sessions a week and each session take 60-minute for three stages. The plasma level of TSH, triiodothyronine (T3), and thyroxin (T4) were measured before and after 12 weeks aerobic exercise. After 12 weeks of intervention, no significant change was observed in the plasma level of TSH and thyroid hormones (T3 and T4) in the exercise group (aerobic exercise). In general, it can be concluded that the aerobic exercise alone had no effect on plasma level of TSH, andthyroid hormones ofT3 and T4.