The purpose of the present study is investigation of influence of a triathlon competition including swimming, bicycling and running on glomerular proteinuria in southern regions of Iran’s triathlon athletes. Seven triathlon athletes of southern region of Iran’s athletes with average age of 22±2 years old, 174±0.039 cm height and 62.42±4.64 Kg weight, participate in a triathlon speed contest including 800 m swimming, 20 km bicycling and 5 km running. Before, immediately after and 45 minutes after the competition, urine samples were gathered. The collected samples were analyzed by using urine electrophoresis method and for each sample; the amount of total protein globulin albumin was gauged. In order to investigate changes of the variant from before to immediately then and to 45 min after the contest, statistical variance analysis approach with repeated measurements and t paired test with bonferroni correction were taken. All of the three under study variants had significant increases from before to immediately after the competition (P<0.05), and decreased significantly to 45 min after the competition (P<0.05). It seems a triathlon contest, which conducted by triathlon athletes of southern regions of Iran, was so intense that cause to a significant and considerable increase in urine excretion of heavy molecular weight proteins. Also, appears after the initial increase, the mentioned amounts came back to the basic values. There isn’t any evidence that urine protein excretion could be a limiting factor in activities of southern regions of Iran’s triathlon athletes. However, rather investigations are required.