mushroom is macro fungus having fleshy fruiting bodies with good source of protein, vitamins and minerals and very low fat and they require minimal sunlight for their growth. Cultivation of mushrooms becomes the most popular agro business worldwide. The growth and nutritional value of oyster mushroom (Pleurotus ostreatus) were determined using different substrates. Substrates such as topsoil(TS), areca nut husk(AH), areca palm leaves(AL), bamboo shoots(BS), and it’s mixture with top soil were used for cultivation. The growth of mycelia on bamboo shoot was observed for a few days and then it was decayed. The highest mycelia growth was observed in topsoil followed by areca nut husk, areca palm leaves, mixture of areca nut husk and topsoil and very less in mixture of bamboo shoot and topsoil and mixture areca palm leaves and topsoil