In order to investigate the effect of salinity stress by influenced of unconventional water at five levels (0, 2, 4, 6, 8 Ds/m) on some morphological parameters and Ajmalicine alkaloid content in two cultivars of Catharanthus roseus Don. (Rosea and Alba) was conducted an experiment as factorial arrangement in completely randomized design with 6 replications in Abadan city, Khuzestan, Iran in the years 2011-2012. Results showed shoot and root length, fresh and dry weight of shoots and roots decreased under salinity conditions. Ajmalicine alkaloid content increased under salinity conditions and Alba cultivar produced an amount less than Rosea. Highest fresh and dry weight of shoots and roots were belonged to Alba and lowest Length of shoot and root to Rosea.