Estimation of residual concentration of Endosulfan toxicity and impact on nitrogenase enzyme activity at different concentrations of Endosulfan was carried out during the study. For identification of the exact effect on rhizobia or nitrogenase activity for N2 fixation, residues of Endosulfan were analyzed. The nitrogenase activity was continued but with increasing concentration of Endosulfan, this activity gets completely stopped. Also different residual concentrations were used to determine the impact of higher concentrations of endosulfan on selected leguminous plant Arichis hypogea. It is observed that the inhibitory effect was observed at (250 ppm) a very low concentration.
