A preliminary study was carried out to investigate the impact of polythene and lime onbees wax baited top bars hives on colonization, hive weight and pest infestation in Ogbomoso (longitude 40301E and latitude 10051N), Nigeria. All hives were baited with bees wax at the rate of 25 g per hive. The experimental treatments were lime (L), Polythene (P), Polythene and Lime (PL) and baiting alone which was the control. The results of the experiment show that none of the treatments prevented hive colonization by bees. Although treatment effect was not significant (p>0.05), the first treatment to be colonized was polythene (P) where hive was colonized 17 days after installation. For lime (L) treatment, Polythene and lime (PL) and control (wax alone) colonization periods were 48, 60 and 74.5 days respectively. Pests encountered were Crematogester species F. (Hymenoptera: Formicidae) and Belonogaster juneus F. (Hymenoleptera: Vespide) in polythene (P) and Polythene and lime (PL) respectively. Hive treated with polythene had greater weight towards the end of the experimental period whereas hive treated with lime had comparatively early colonization suggesting that lime could be a synergist to wax in baiting of hives