Aerva lanata (Linn.) Juss. ex Schult (Family-Amaranthaceae) is an medicinally important plant known for its prolonged hypertensive and hypoglycemic activity. This study is focused on standardization of in vitro propagation and phytochemical studies of A. lanata. Direct organogenesis of shoots from nodal segments was achieved by culturing on MS medium supplemented with different concentrations and combinations of growth regulators BAP (0.5–2.5 mg/l), IAA (0.5–2.5 mg/l) and KIN (0.2–1.0 mg/l). The maximum number of multiple shoots per explants was obtained in the combination BAP and (1.5 mg/l) and KIN (0.6 mg/l). Half and full strength of MS medium with different concentrations of auxins such as IAA and IBA, were used for in vitro root formation. IAA (1.5 mg/l) with half strength MS medium supported the greatest increase in the number of average roots. Hardening of regenerated plants was achieved with on 1:2:1 ratio of sterilized garden soil, red soil, compost. In the current investigation, 70% of A. lanata plantlets survived transfer to ex situ conditions.
