The present study evaluate the effect of a column chromatographic fraction (F6) obtained from nbutanol soluble portion of ethanolic extract of Cassia tora L. seeds on biochemical parameters and on β-cells regeneration in alloxan induced diabetic rats. Different doses (0.1, 0.25, and 0.5 g/kg body wt.) of fraction F6 were administered orally to different groups of normal or alloxan induced diabetic rats either as single dose daily or multiple dose spanning over two weeks. Levels of glucose, triglycerides, urea, uric acid, creatinine, asparate amino transferase (AST) and alanine amino transferase (ALT) in blood or serum were determined. Histopathological study was also carried out to evaluate the effect of fraction F6 on diabetic pancreas. Oral administration of the fraction F6 significantly decreased blood glucose level in normal and alloxan induced diabetic rats both in single dose as well as multiple dose experiment when compared with control. A significant (p<0.05) reduction was observed in total cholesterol, triglycerides, asparate amino transferase (AST) and alanine amino transferase (ALT) in serum to the control levels in diabetic animals. The increased serum urea, uric acid and creatinine concentration were observed in diabetic rats, decreased significantly following administration of F6. The present observations suggested that the fraction F6 obtained from Cassia tora L seed decreased elevated blood glucuose levels and markers of hepatic and renal dysfunctions in diabetic rats. These effects might be due regenerating influence on existing beta cells of islets and possibly some other unknown mechanism of action.
