Banana plantains are grown as staple food, significant cash crops and major export crops in many tropical and subtropical countries. The objective of this study was to assess in vitro regeneration response of Poovan and Monthan varieties of banana using low cost macronutrients source to reduce the cost of micro-propagule production. The five MS conventional macronutrients;viz, ammonium nitrate, potassium nitrate, calcium chloride, magnesium sulphate, and potassium hydrogen phosphate were substituted to the locally available urea fertilizer, potassium fertilizer, cal rich, magnesium sulphate fertiliser and trio powder respectively. The result show that the sword suckers banana shoots and roots compared to conventional medium significant differences (p>00.5). The use of locally available macronutrients significantly (p<0.05) reduced the cost of micropropagation of banana. The Poovan performed better the highly shoots and roots produced and compared to Monthan. There was 72.4 % savings in the cost of the macronutrients used in media preparation.
