Lipopolysaccharide (LPS) located in the cell wall of most gram-negative bacteria known to have a variety of biological and immunochemical activities. Fibroblasts are spindle shaped cells found in the majority of tissues and organs of the body associated with extracellular matrix (ECM) molecules. The aim of this study was to determine the effect of LPS extracted from Salmonella enteritidis on hydrogen peroxide production and total antioxidant capacity (TAC) in fibroblast cells. In present study LPS was extracted from Salmonella enteritidis cell wall by methanol chloroform method and purified. Electrophoresis and pyrogenicity test for purity of extracted sample was done. Electrophoresis was performed on extracted LPS with standard LPS in agarose 12% gel and was stained by AgNO3. Pyrogenicity of LPS was tested in rabbit before injection, 30 minutes after injection and 1 hour after injection. In this assess we used respectively 75, 100, 125 ng of LPS combination extracted from salmonella enteritidis in MTT test for cytotoxic level measurement on fibroblast cells. 100 ng of LPS was used for assay of hydrogen peroxide and TAC in human fibroblast cells. Human fibroblast cells treated with 100 ng LPS along with non-treated cells were incubated. Our study revealed that LPS can stimulate the increasing of hydrogen peroxide and decreasing of TAC in human fibroblast cells. These finding also confirmed that extracted LPS by methanolchloroform method has the same activity of standard LPS.
