The experiment was conducted at ASPEE, Agricultural Research and Development Foundation Lab, Village Nare, Taluka Wada, District Thane, Maharashtra during Rabi 2013-14. In this study the effects of different levels of sugar in pulsing treatments on post harvest quality of gladiolus cv. American Beauty was carried out. For long-distance transportation, an even earlier harvest stage can be recommended, if it is combined with sugar pulsing (20 % for 20hrs.) to ensure proper opening of the flowers at their destination market. The treatment 20% sugar results in a greater number of opened flowers, marketable condition of spike and longer vase life of spike in days for the gladiolus cv. American Beauty. The parameter of vase life was studied indicated that, the sugar levels increased ultimately increasing the greater number of opened flowers per spike over control