Four feeding trials were conducted to investigate the effects of using different levels of alfalfa extract on performance, egg quality, blood biochemical and immunity parameters of laying hens. This study was carried out with 500of Hy- line (W36) hens in 4 treatment groups and 5 repetitions and 25 hens for each group. The groups were follow: E1) First group as control group did not receive any herbal planet oil, E2) E3) and E4) 100 ppm, 150 ppm and 200 ppm of alfalfa extractrespectively. The highest percent of production percent andMass production were in group 4 but the best result for FCR was in the E3.The highest level for special weight and yolk index were in E4 also the shell weight were increase in E3.Alsothe serum total cholesterol and Triglycerides concentration were significantly reduced in group of 4 compared to the control group (P<0.05). But the concentration of serum HDL and LDL were not significantly reduce in groups compared to the control group. Butdid not show significantly change on the immune system of laying hens.
