Cellulase activity under different cultural parameters by Pseudomonas putida, Bacillus subtilis, Bacillus lichenformis I and Bacillus licheniformis II in liquid medium was studied. The temperature of the enzyme activity was monitored for each of the Isolates. The pH and effect of different nitrogen sources were also determined. Temperature was observed to influence cellulase activity significantly. Cellulase activity was observed to be highest for Bacillus subtilis at 28°C with a value of 0.49mg/ml while Bacillus licheniforms I and II had the highest activity at 40oC with a value of 0.52mg/ml and 0.44mg/ml respectively. Optimal activity for Pseudomonas putida was observed at 320C with a value of 0.51mg/ml. Cellulase activity was highest at pH 7.0 on day 7 in all the Bacteria isolate except Bacillus licheniformis I whose highest production of 0.43mg/ml was found in pH 5.5. When different Nitrogen sources were used, Peptone was observed to induce highest cellulase activity with value of reducing sugar of 0.81mg/ml on day 4 in Bacillus licheniformis II. Bacillus subtilis has highest cellulase activity in yeast extract, urea and NaNO2. This study shows the need to determine best cultural conditions for optimal cellulase activity of bacteria isolate. This will enhance their enzyme production.
